线粒体自噬研究利器

 

线粒体自噬

 

线粒体自噬的研究是目前生物学领域的研究热点, 汉恒生物自主研发的线粒体特异性定位荧光探针(HBAD-Mito-dsred)可准确定位标记线粒体,结合汉恒独家推出的双荧光LC3细胞自噬腺病毒的使用,即可准确实时地追踪线粒体自噬的动态过程。


 

XIANLITIZISHI(mitophagy)SHIZHIXIBAOTONGGUOZISHIDEJIZHIXUANZEXINGDIQINGCHUXIANLITIDEGUOCHENG。XUANZEXINGQINGCHUSHOUSUNSHANGHUOGONGNENGBUWANZHENGDEXIANLITIDUIYUZHENGGEXIANLITIWANGLUODEGONGNENGWANZHENGXINGHEXIBAOSHENGCUNLAISHUOSHIFENGUANJIAN。XIANLITIZISHIDEYICHANGHEHENDUOJIBINGMIQIEXIANGGUAN, YINCIDUIYUXIANLITIZISHIDEJUTIFENZIJIZHIYIJISHENGLIYIYIYANJIUYOUHENZHONGYAODESHENGWUXUEYIYI。

 

线粒体自噬的信号通路:

 

1) Pink/Parkin pathway         

2) Bnip3/Nix pathway  

3) FUNDC1 pathway         

 

                    

 Mitophagy pathway: Pink1/Parkin OR Bnip3/Nix

   

XIANLITIZISHIDEYICHANGHEHENDUOJIBINGMIQIEXIANGGUAN, YINCIDUIYUXIANLITIZISHIDEJUTIFENZIJIZHIYIJISHENGLIYIYIYANJIUYOUHENZHONGYAODESHENGWUXUEYIYI。HANHENGSHENGWUJINGYANFENGFUDEXIANLITIZISHIYANJIUTUANDUI,WEININTIGONGXIANLITIZISHIYANJIUFANGANYUYANJIUGONGJU。

 

 

线粒体自噬研究工具与研究方法:

 

汉恒生物有多种线粒体自噬病毒研究工具可以提供,便于直接感染目的细胞后直观地观察线粒体自噬的变化

 

 

一、汉恒线粒体自噬表型研究工具

 

1)Ad-GFP-LC3XIANBINGDUBINGDUXITONG,KEGAOXIAOGANRANMUDEXIBAO,BIAODAGFP-LC3,GANRANGANRANHOUXIBAOKEZAIYINGGUANGXIANWEIJINGXIASHISHIGUANCHAZISHIDEZHENGTISHUIPING;

 

2)Ad-HBmTur-Mito腺病毒系统,为汉恒生物自主研发的线粒体特异性定位荧光探针(HBAD-Mito-dsred)可准确定位标记线粒体,结合汉恒独家推出的双荧光LC3细胞自噬腺病毒的使用,即可准确实时地追踪线粒体自噬的动态过程;

 

SHIYONGFANGFA:Ad-GFP-LC3+HBAD-Mito-dsredGONGGANRANMUDEXIBAO,confocalJIANCESHUANGYINGGUANGGONGDINGWEIDEQINGKUANG,RUGUOGONGDINGWEI,ZECUNZAIXIANLITIZISHI!

 

 

 

 

二、汉恒线粒体自噬通路研究工具

 

1)Ad-Parkin-EGFP

2)Ad-Bnip3-EGFP+Ad-Nix-EGFP

3)Ad-FUNDC1-EGFP

 

SHIYONGFANGFA:YUHANHENGHBAD-Mito-dsredDINGWEIXIANLITIGONGGANRANMUDEXIBAO,confocalJIANCEGONGDINGWEIQINGKUANG,JIANBIEXIANGGUANXINHAOFENZIDEXIANLITIZHUANWEI!

 

 

CHUCIZHIWAI,WOMENHAIYONGYOUCANYUZISHIDECHANGJIANJIYINDEXIANBINGDUXIANHUOBeclin1、Atg7DENGMANZUNINSHIYANDEXUQIU。

 

 

参考文献

  1、TRB3 links insulin IGF to tumour promotion by interacting with p62 and impeding autophagic proteasomal degradations;

     (2016NIANFABIAOYUNature Communications,ZUOZHE:HUZHUOWEI)

 

 

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