qPCR背景介绍

ZAISHISHIYINGGUANGDINGLIANGPCRZHONG，DUIMEIYILUNXUNHUANDEYINGGUANGXINHAOQIANGDUJINXINGJIANCEBINGJILU，SUIZHEFANYINGDEJINXING，JIANCEDAODEYINGGUANGXINHAOKEYIHUIZHICHENGYITIAOQUXIAN，JIWEIKUOZENGQUXIAN。YIBANERYAN，KUOZENGQUXIANKEYIFENCHENGSANGEJIEDUAN：JIXIANQI（Baseline phase），ZHISHUZENGZHANGQI（Log-linear phase）HEPINGTAIQI（Plateau phase）（TUYI）。ZAIKUOZENGZAOQIJIJIXIANQI，KUOZENGDEYINGGUANGXINHAOBEIYINGGUANGBEIJINGXINHAOSUOYANGAI，WUFAPANDUANCHANWULIANGDEBIANHUA。ZAIPINGTAIQI，KUOZENGCHANWUYIBUZAICHENGZHISHUJIDEZENGJIA，PCRDEZHONGCHANWULIANGYUQISHIMOBANLIANGZHIJIANWUXIANXINGGUANXI，SUOYIGENJUZUIZHONGDEPCRCHANWULIANGBUNENGJISUANCHUCHUSHIMOBANLIANG。ZHIYOUZAIYINGGUANGXINHAOZHISHUKUOZENGJIEDUAN，PCRCHANWULIANGDEDUISHUZHIYUQISHIMOBANLIANGZHIJIANCUNZAIXIANXINGGUANXI，KEZAICIJIEDUANJINXINGDINGLIANGFENXI。

流程图

汉恒提供服务天博电竞

HANHENGSHENGWUTIANBODIANJING（SHANGHAI）YOUXIANTIANBODIANJINGTIGONGCONGYINWUSHEJI，XIBAOHUOZHEZUZHIYANGPINCHULI，qPCRJIANCEYIJIWANZHENGDESHUJUBAOGAODENGFUWU。WEININTIGONGZUIYOUZHIDEFUWU，WEININDEKEYANBAOJIAHUHANG。

KEHUTIGONG：

1.细胞：细胞数10⁶-10⁷；组织：总量>100mg；DNA或RNA样品：总量>2 μg

2.BAJIYINXINXI：BAOKUOJIYINXULIEHUOGenBank DENGLUHAODENG

3.BEIJINGZILIAO：BAOKUOSHENGWUWUZHONGXINXI、JIYINGONGNENG、YONGTU、TEXING、DNA/RNA LAIYUANDENG

（BEIZHU：QINGZAISONGYANGQIAN，YUJISHUQUERENJUTISONGYANGQINGKUANG。）

实例展示：

（Hou Z ,Qin X ,Hu Y ,et al. 2019）

ZAIYANJIUZHANGQIDUANLIANBAOHUXINZANGDEFENZIJIZHIZHONG，YANJIURENYUANDUIYUNDONGDASHU(Exe-exo)HEJIUZUODASHU(Sed-exo)DEWAIMITIJINXINGmiRNA-seqHEqPCR，LAIQUEDINGYANJIUMUBIAOYUXINZANGBAOHUXIANGGUANDEmiR-342 5p。

CANKAOWENXIAN：

Hawkins, S. F. C, Guest, P. C. Multiplex Analyses Using Real-Time Quantitative PCR[J]. Methods in Molecular Biology,2016,1546:125-133.

Wong M L , Medrano J F . Real-time PCR for mRNA quantitation[J]. BioTechniques, 2005, 39(1):75-85

Hou Z , Qin X , Hu Y , et al. Longterm Exercise-Derived Exosomal miR-342-5p: A Novel Exerkine for Cardioprotection[J]. Circulation Research, 2019,124(9):1386-1400.