实验原理
YINGHUOCHONGYINGGUANGSUMEI(firefly luciferase, fluc)NENGCUIHUAYINGGUANGSU(luciferin)CHANSHENGSHENGWUYINGGUANGFANYING,FANYINGGUOCHENGRUXIATUSUOSHI。
特点:
1.DIWUCUNZAISHI,CUIHUADIWUFACHUYINGGUANG
2.YINGGUANGLIANGDUHEYINGGUANGSUMEIDELIANGCHENGZHENGBI
GENJUYINGGUANGQIANGDU,FANYINGBIAODAZAITISHANGF-lucDEBIAODALIANG
应用
转录因子结合位点与启动子活性分析
ZHUANLUYINZI(Transcription factors,TF)SHIYIZHONGJUYOUTESHUJIEGOU、XINGSHIDIAOKONGJIYINBIAODAGONGNENGDEDANBAIZHIFENZI,YECHENGWEIFANSHIZUOYONGYINZI,ZHUANLUYINZIJINYUQIBAQIDONGZIZHONGDETEYISHUNXUJIEHE,CONGERZENGQIANGQIXIAYOUJIYINDEBIAODA。
YINGGUANGSUMEIBAOGAOJIYINSHIYANSHIJIANCEZHEILEIZHUANLUYINZIJIQIBAQIDONGZIZHONGTEYIXULIEJIEHEDEZHONGYAOSHOUDUAN
产品规格
实验步骤
YI、PEIZHISHIJI
1.1 Lysis Buffer:Lysis Buffer (5X) YONGSHUIXISHI5BEI,PEIZHICHENGGONGZUOYE;
1.2. Luciferase Reaction Reagent:YONGLuciferase Reaction Buffer(5 ml)RONGJIELuciferase Reaction SubstrateGANFEN,HUNYUNHOUFENZHUANG、BIGUANGBAOCUN,BIMIANFANFUDONGRONG。
ER、LIEJIEXIBAO
2.1 XIBAOLIEJIESHIMIDUYINGXIAOYU95%,XIBAOSHENGZHANGGUOMI,YIDAOZHILIEJIEBUCHONGFEN;ZHILISHUNZHUAN、BINGDUGANRANXIBAOHOU,XUPEIYANGYIDUANSHIJIAN(YIBAN48XIAOSHI),SHIYINGGUANGSUMEICHONGFENBIAODA;DIAOJIESHIYANCANSHU,YIMANZUSHANGSHUTIAOJIAN;
2.2 QUCHUPEIYANGJI,YONGPBSRUNXIYIBIAN,ZHUYIBUYAOJIANGXIBAOCHUIQI;JIARUSHILIANGDELysis Buffer。
2.3 JIANGXIBAOPEIYANGBANZHIYUYAOCHUANGSHANG,SHIWEN、QINGHUANGFUYU15FENZHONG;
2.4 JIANGLIEJIEWUZHUANYIDAOXINDEEPGUANZHONG,4oC、12,000rpmLIXIN1FENZHONG,QUSHANGQINGBEIYONG
SAN、YINGGUANGJIANCE
QU20μlLIEJIEWU,JIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent,HUNYUNHOUYUMEIBIAOYIZHONGJIANCEfLucXINHAO。
HANHENGYINGGUANGSUMEI(firefly)JIANCESHIJIHEJUYOUCAOZUOJIANDAN、JIANCEKUAIJIE、LINGMINDUGAODENGYOUDIAN,SHIKEYANGONGZUODEYOUZHIGONGJU!
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