实验原理

 

 

YINGHUOCHONGYINGGUANGSUMEI（firefly luciferase, fluc）NENGCUIHUAYINGGUANGSU（luciferin）CHANSHENGSHENGWUYINGGUANGFANYING，FANYINGGUOCHENGRUXIATUSUOSHI。

 

 

特点：

1.DIWUCUNZAISHI，CUIHUADIWUFACHUYINGGUANG

2.YINGGUANGLIANGDUHEYINGGUANGSUMEIDELIANGCHENGZHENGBI

 

 

GENJUYINGGUANGQIANGDU，FANYINGBIAODAZAITISHANGF-lucDEBIAODALIANG

 

 

应用

 

转录因子结合位点与启动子活性分析

 

ZHUANLUYINZI（Transcription factors，TF）SHIYIZHONGJUYOUTESHUJIEGOU、XINGSHIDIAOKONGJIYINBIAODAGONGNENGDEDANBAIZHIFENZI，YECHENGWEIFANSHIZUOYONGYINZI，ZHUANLUYINZIJINYUQIBAQIDONGZIZHONGDETEYISHUNXUJIEHE，CONGERZENGQIANGQIXIAYOUJIYINDEBIAODA。

YINGGUANGSUMEIBAOGAOJIYINSHIYANSHIJIANCEZHEILEIZHUANLUYINZIJIQIBAQIDONGZIZHONGTEYIXULIEJIEHEDEZHONGYAOSHOUDUAN

 

 

产品规格

 

 

实验步骤

 

YI、PEIZHISHIJI

1.1 Lysis Buffer：Lysis Buffer (5X) YONGSHUIXISHI5BEI，PEIZHICHENGGONGZUOYE；

1.2. Luciferase Reaction Reagent：YONGLuciferase Reaction Buffer（5 ml）RONGJIELuciferase Reaction SubstrateGANFEN，HUNYUNHOUFENZHUANG、BIGUANGBAOCUN，BIMIANFANFUDONGRONG。

 

ER、LIEJIEXIBAO

2.1 XIBAOLIEJIESHIMIDUYINGXIAOYU95%，XIBAOSHENGZHANGGUOMI，YIDAOZHILIEJIEBUCHONGFEN；ZHILISHUNZHUAN、BINGDUGANRANXIBAOHOU，XUPEIYANGYIDUANSHIJIAN（YIBAN48XIAOSHI），SHIYINGGUANGSUMEICHONGFENBIAODA；DIAOJIESHIYANCANSHU，YIMANZUSHANGSHUTIAOJIAN；

2.2 QUCHUPEIYANGJI，YONGPBSRUNXIYIBIAN，ZHUYIBUYAOJIANGXIBAOCHUIQI；JIARUSHILIANGDELysis Buffer。

2.3 JIANGXIBAOPEIYANGBANZHIYUYAOCHUANGSHANG，SHIWEN、QINGHUANGFUYU15FENZHONG；

2.4 JIANGLIEJIEWUZHUANYIDAOXINDEEPGUANZHONG，4oC、12,000rpmLIXIN1FENZHONG，QUSHANGQINGBEIYONG

 

SAN、YINGGUANGJIANCE

QU20μlLIEJIEWU，JIARU100μlPINGHENGZHISHIWENDELuciferase Reaction Reagent，HUNYUNHOUYUMEIBIAOYIZHONGJIANCEfLucXINHAO。

 

HANHENGYINGGUANGSUMEI（firefly）JIANCESHIJIHEJUYOUCAOZUOJIANDAN、JIANCEKUAIJIE、LINGMINDUGAODENGYOUDIAN，SHIKEYANGONGZUODEYOUZHIGONGJU!