双萤(荧)光素酶检测试剂盒

 

双萤光素酶检测试剂盒

 

YINGGUANGSUMEI(Luciferase) :SHIZIRANJIEZHONGNENGGOUCHANSHENGSHENGWUFAGUANGDEMEIDETONGCHENG,QIZHONGZUIYOUDAIBIAOXINGDESHILAIZIYINGHUOCHONGTINEI(Firefly)HEHAISHEN(Renilla)TINEIDELIANGLEIYINGGUANGSUMEI,YINGGUANGSUMEIKEYICUIHUATEDINGDEYINGGUANGSUDIWUZHUANBIANCHENGYANGYINGGUANGSU,TONGSHIFACHUQIANGLIEDEGUANG。

 

YINGHUOCHONGYINGGUANGSUMEI(firefly Luciferase,F-Luc), 61kDa,KECUIHUAD-YINGGUANGSUFACHUYINGGUANG

HAISHENYINGGUANGSUMEI(Ranilla luciferase,R-Luc), 36kDa,KECUIHUAQIANGCHANGSUFACHUYINGGUANG

 

 


 

 

特点

1.DIWUCUNZAISHI,CUIHUADIWUFACHUYINGGUANG

2.YINGGUANGLIANGDUHEYINGGUANGSUMEIDELIANGCHENGZHENGBI

 

 

应用

 

miRNA和靶基因结合双萤光素酶验证方案

 

miRNASHIYILEIYOUNEIYUANJIYINBIANMADEZHANGDUYUEWEI22 GEHEGANSUANDEFEIBIANMADANLIANRNAFENZI,TONGGUOBAXIANGJIEHEMUDEJIYINDEJIEHEWEIDIAN(TONGCHANGWEIYUJIYINDE3DUANFEIBIANMAQU——3UTRQU),YIZHIQISHANGYOUJIYINDEBIAODA。

JIANYUmiRNADETEDIANJIlucDETEXING。

 

miRNAJIQIBAJIEHEWEIDIANDEYANZHENGKEYITONGGUOSHUANGYINGGUANGSHIYANLAISHIXIAN,BAOKUO:

● miRNAYUQIBADIANXIANGGUANFENXI

● miRNABAJIYINYANZHENG

● lncRNAYUcircRNA XIFUmiRNAYANZHENGDENG

 


 

 

检测原理

 

BAmiRQIANZAIJIEHEGOUJIANDAOBAOGAOZAITISHANGF-LuciferaseXIAYOU,JIANGBAOGAOZAITIYU miRDEmimicsGONGTONGZHUANRANDAOXIBAONEICEDINGYINGGUANGSUMEIZHI,FENXIF-LuciferaseHUOXINGJIKEFANYINGmiRNAYUJIEHEWEIDIANDEJIEHETEXING

 


 

检测方法及载体工具

 

BAmiRQIANZAIJIEHEWEIDIANSHANGXIAYOU~500bp(he/HUOTUBIANWEIDIAN)GOUJIANDAOYINGGUANGSUMEIBAOGAOZAITISHANGpsiCHECK2DEF-LuciferaseXIAYOU3UTRQU,ZAITISHANGCHIXUXINGBIAODAR-LuciferaseYUANJIANZUOWEINEICAN, YUmiRDEmimics(XIAOPIANDUANSHUANGLIANmiRNA )/ NCGONGTONGZHUANRAN293TXIBAOGONGTONGZHUANRANDAOXIBAONEICEDINGYINGGUANGSUMEIZHI,FENXIF-LuciferaseHUOXINGJIKEFANYINGmiRNAYUJIEHEWEIDIANDEJIEHETEXING

 


miRNAJIBADIANYANZHENGF-LuciferaseBAOGAOZAITI

 

 

产品规格

 

 

 

 

实验步骤

 

YI、XIBAOZHUANRAN

 ZHUANRAN48 hHOUSHOUJIYANGJIANCE。

 

ER、YINGGUANGJIANCE

1.      JIANGPEIYANGXIANGZHONGDE24KONGBANQUCHU,FANGZAISHIWEN,ZHIXIBAOPINGHENGDAOSHIWEN。

2.      JIARUXIBAOLIEJIEYELysis Buffer,CHONGFENLIEJIEHOU12000rpm 4℃LIXIN10minBINGZHUANYIZHI96KONGBANZHONG。

3.      MEIKONGJIARUF-LucDIWUSHIJI,FANGRUMEIBIAOYIZHONGCEDINGJILUFirefly luciferaseZHI。

4.      MEIGEKONGZHONGJIARUStop solutionZHONGZHIF-LucFANYINGBINGCUIMIEF-LucYINGGUANG

5.      MEIKONGJIARUR-LucDIWUFANGRUMEIBIAOYIZHONGCEDINGJILURenillaluc luciferaseZHI

6.      JISUANFirefly luciferaseXIANGDUIYINGGUANGZHIF-Luc/ R-Luc

 

 


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