原代T细胞的基因操作利器
T细胞及其特性简介
TXIBAO(T cell、TLINBAXIBAO/T lymphocyte)SHIDEYIZHONG,ZAIZHONGBANYANZHEZHONGYAODEJIAOSE。TXIBAOZAIXIONGXIANNEIFENHUACHENGSHU,CHENGSHUHOUYIJUYUZHOUWEIZHONG。SUIZHEXIBAOMIANYIZHILIAODERIYISHENRU,TXIBAODEJIYINGONGCHENGHUAGAIZAOXIANDEYUELAIYUEZHONGYAO。
RANHOU,TXIBAODEJIYINCAOZUOQUEYICHANGKUNNAN,PUTONGDEDNAZHILIHENNANZHUANRAN,JISHISHIBINGDUJIEDAODEJIYINZHUANRANCAOZUO,YEYOUBUSHAONANTI,ZHUYAOTIXIANZAIJIGEFANGMIAN:
1.CHANGGUIDEMANBINGDU、NIZHUANLUBINGDU,YOUYU1)TXIBAOMOSHANGSHOUTIDEBUWENDING;2)FEIJIHUODETXIBAOBENSHENWUZENGZHINENGLI;3)TXIBAONEIBUYOUZUAIFANZHUANLUDEJIZHIDENGYUANYINERDAOZHIMANBINGDUHENIZHUANLUBINGDUDETXIBAOGANRANNENGLIPIANRUO,XUYAODUOCIZHONGFUGANRAN,QIEDUIYUSHIYANCAOZUODETIAOJIANYAOQIUSHIFENKEKE。
2.TIWAIPEIYANGDETXIBAOBENSHENSHIFENMINGANQIECUIRUO,MANBINGDUGANRANDUIXIBAODEZHUANGTAIYINGXIANGFEICHANGDA。
3.TXIBAOSHIXUANFUXIBAO,JINYIBUZENGDALEBINGDUYOUXIAODENANDU。
汉恒原代T细胞感染实例——使用悬浮细胞专用腺病毒ads感染
悬浮细胞专用腺病毒Ads感染小鼠原代CD4+ T细胞
XIBAO:XIAOSHUYUANDAICD4+ TXIBAO
CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48h
BINGDU:Ads-GFP, 1×10^10 PFU/ml
SHIYONGFANGSHI:MOI=500
悬浮细胞专用腺病毒Ads感染人原代CD4+ T细胞
XIBAO:RENYUANDAICD4+ TXIBAO
CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48h
BINGDU:Ads-GFP, 1×10^10 PFU/ml
SHIYONGFANGSHI:MOI=100
汉恒原代T细胞感染实例——使用慢病毒和逆转录病毒感染
原代T细胞专用病毒-慢病毒感染人原代CD4+ T细胞
XIBAO:RENYUANDAICD4+ TXIBAO
CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48h
BINGDU:hTLv-GFP,1*10^8 TU/ml
SHIYONGFANGSHI:MOI=50
原代T细胞专用病毒-逆转录病毒感染小鼠原代CD4+ T细胞
XIBAO:XIAOSHUYUANDAICD4+ TXIBAO
CIJIFANGSHI:CD3/CD28KANGTIHEIL-2JIHUO48 h
BINGDU:TXIBAOZHUANYONGNIZHUANLUBINGDUmTRv-GFP,1×108 TU/ml
SHIYONGFANGSHI:MOI=100
参考文献
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