产品简介

YANJIUMOUGEJIYINDEGONGNENGZUICHANGYONGDESHOUDUANSHIZAISUZHUXIBAOZHONGGUOLIANGBIAODAHUOZHETONGGUORNAGANRAODEFANGFAknock-downGAIJIYIN，CHANGGUISHOUDUANYOUSHUNSHIZHUANRANHESHAIXUANWENDINGXIBAOXI。

SHAIXUANCHUGAIJIYINDEGUOBIAODAHUOZHERNAGANRAODEWENDINGXIBAOXIHUIJININDESHIYANDAILAIJIDADEBIANLI。YOULEWENDINGXIBAOXI，HOUQIDECo-IPHUOZHEPull-DownSHIYANHUIFEICHANGBIANLI；WENDINGBIAODAZHONGZUYINGGUANGDANBAIDEXIBAOXIKEYIRANGNINDONGTAIDEGUANCHAFENZIZAIXIBAOZHONGDEYUNDONG。 GOUJIANWENDINGXIBAOXIDEJIBENYUANLISHIJIANGWAIYUANDNAKELONGDAOJUYOUMOUZHONGKANGXINGDEZAITISHANG，ZAITIBEIZHUANRANDAOSUZHUXIBAOBINGZHENGHEDAOSUZHURANSETIZHONG，YONGZAITIZHONGSUOHANDEKANGXINGBIAOZHIJINXINGSHAIXUAN。ZUICHANGYONGDEZHENHEBIAODAZAITIDEKANGXINGSHAIXUANBIAOZHIWUYOUXINMEISU（neomycin）、CHAOMEISU（hygromycin）HEPIAOLINGMEISU(puromycin)，CHANGYONGG418LAIDAITIXINMEISUJINXINGXUANZEXINGSHAIXUAN，SHAIXUANDEDAOKEWENDINGBIAODAMUDEDANBAI，HUOZHEWENDINGBIAODACHENMOTEDINGJIYINDEXIBAOZHU。

筛选稳定细胞系的方法

1）ZHUANRANZHILIHOU，DANKELONGFANGFASHAIXUANWENDINGXIBAOXI

2）MANBINGDUGANRANSHAIXUANWENDINGXIBAOXI

MANBINGDUGANRANFANGFAJIAOZHILIZHUANRANSHAIXUANDANKELONGFANGFAGENGFANGBIANHEGAOXIAO，SHIMUQIANZHULIUDEWENDINGXIBAOXISHAIXUANFANGFA。MANBINGDUJIHUKEYIGANRANSUOYOUZHONGLEIDEXIBAO，BINGQIEZAIGANRANHOUKEYIZHENGHEDAOSHOUGANRANXIBAODEJIYINZU，JINXINGZHANGSHIJIANDEWENDINGBIAODA，YINCI，MANBINGDUCHANGYONGYUZHIBEIWENDINGBIAODA/CHENMOTEDINGJIYINDEDANKELONGXIBAOZHU。

HANHENGSHENGWUGOUJIANBAOZHUANGDEDAIpuromycinKANGXINGDEMANBINGDU，ZAIGANRANMUDEXIBAOHOU，KETONGGUOJIARUpuromycinJINXINGXUANZEXINGSHAIXUAN，CONGERHUODEWENDINGBIAODAshRNAHUOZHETEDINGJIYINDEXIBAOZHU。WOMENTIGONGBINGDUBAOZHUANGFUWU（NINKEYIZIJIWANCHENGWENTAIXIBAOSHAIXUAN），HUOZHEYIZHANSHIDEWENDINGXIBAOXIDESHAIXUANFUWU。

服务流程

我们的优势

●　种子优良-- 严 选低代数细胞来源，无支原体无黑胶虫；

HANHENGSHENGWUSUOYOUDEXIBAOJUNCONGZHONGKEYUANXIBAOKUGOUMAI，DAISHUDIWUWURAN，KETIGONGSTRJIANCEBAOGAO

●　性状稳定-- 严 酷的筛选及靶基因表达验证体系；

HANHENGSHENGWUSUOYOUDEWENZHUANZHUDOUCAIYONGRT-qPCRYANZHENG，BAOZHENGBIAODASHUIPING

●　活力无损-- 严 格的建系后连续3代细胞增殖活力评价