简介：

 

lncRNADEZUOYONGJIZHIYOUHENDUO，YIceRNA（competing endogenous RNA，JINGZHENGXINGDENEIYUANRNA）XINGSHIFAHUIGONGNENGSHIQIZHONGDEYIZHONG，JIlncRNAYUBAJIYINDE3’UTRJINGZHENGJIEHEXIANGTONGDEmicroRNA，JIANGDImicroRNADUIBAJIYINDEYIZHIGONGNENG，CONGERSHANGDIAOBAJIYINDEBIAODALIANG。WEILEJIANCEQIXIAOGUO，WOMENKEYIYONGWestern BlotJIANCEBADANBAIDEBIAODASHUIPING，YEKEYIYONGqRT-PCRJIANCEBAJIYINDEBIAODA。DANSHIZHEILIANGZHONGFANGSHIDOUWUFAQUEDINGlncRNASHIFOUTONGGUOceRNAFANGSHIDIAOKONGBAJIYINDEBIAODA。YINCI，WOMENKEYITONGGUO“microRNAYUlncRNAJIEHEYANZHENG”，“microRNAYUBAJIYINJIEHEYANZHENG”HE“lncRNAYUBAJIYIN3’UTRJINGZHENGJIEHEmicroRNAYANZHENG”SHIYAN，GOUJIANSensor reporterBINGJINXINGHUOXINGJIANCE，DUIlncRNA，microRNAYUBAJIYINZHEISANZHEJIANDEZUOYONGJIZHIZUOCHUZHUNQUEPANDUAN。

 

 

服务优势：

 

（1）LIYONGZHUANYERUANJIANJINXINGmiRNAYUlncRNAHEBAJIYIN3’UTRQUDEJIEHEWEIDIANYUCE；

（2）CAIYONGPromegaTIANBODIANJINGTEYOUpsiCHECKZHILI；

（3）SUOCAIYONGDELuciferaseSHUANGYINGGUANGBAOGAOXITONG，KEYIHUODEKUANFANDEXIANXINGDINGLIANGFANWEIYUJIGAODELINGMINDU；

（4）CAIYONGSHUNSHIZHUANRANJISHU，CAOZUOJIANBIAN、KUAISU，ZHOUQIDUAN，SHIYONGYUJUEDADUOSHUXIBAOLEIXING。

 

 

服务流程：

 

YI、microRNAYUlncRNAJIEHEYANZHENG

（1）ZHIJIEHECHENGBAlncRNAXULIEHUOmicroRNAZAIBAlncRNASHANGDEJIEHEWEIDIANXULIE；

（2）JIANGSHANGSHUHECHENGDEDNAPIANDUANKELONGZHIBAOGAOJIYINZAITIpsiCHECKZHILI；

（3）GOUMAImicroRNA mimics；

（4）JIANGBAOGAOZAITIYUmicroRNA mimicsGONGZHUANRAN293TXIBAO；

（5）DUOGONGNENGMEIBIAOYIJIANCEBAOGAOJIYINDEBIAODASHUIPING；

（6）SHUJUFENXI，ZHENGLITIJIAOKEHUYUANSHISHUJUJITIANBODIANJINGBAOGAO；

 ZHU：WEILESHIYANDEYANJINXING，KEJIARUBAlncRNAXULIEZHONGmicroRNAYUCEJIEHEQUYUDETUBIANTIZUOWEIDUIZHAO，JINYIBUSHUOMINGWENTI。

 

ER、microRNAYUBAJIYINJIEHEYANZHENG

（1）ZHIJIEHECHENGBAJIYIN3’UTRXULIEHUOmicroRNAZAIBAJIYIN3’UTRSHANGDEJIEHEWEIDIANXULIE；

（2）JIANGSHANGSHUHECHENGDEDNAPIANDUANKELONGZHIBAOGAOJIYINZAITIpsiCHECKZHILI；

（3）GOUMAImicroRNA mimics；

（4）JIANGBAOGAOZAITIYUmicroRNA mimicsGONGZHUANRAN293TXIBAO；

（5）DUOGONGNENGMEIBIAOYIJIANCEBAOGAOJIYINDEBIAODASHUIPING；

（6）SHUJUFENXI，ZHENGLITIJIAOKEHUYUANSHISHUJUJITIANBODIANJINGBAOGAO；

 ZHU：WEILESHIYANDEYANJINXING，KEJIARUBAJIYIN3’UTRQUmicroRNAYUCEJIEHEQUYUDETUBIANTIZUOWEIDUIZHAO，JINYIBUSHUOMINGWENTI。

 

SAN、lncRNAYUBAJIYIN3’UTRJINGZHENGJIEHEmicroRNAYANZHENG

（1）ZHIJIEHECHENGBAJIYIN3’UTRXULIEHUOmicroRNAZAIBAJIYIN3’UTRSHANGDEJIEHEWEIDIANXULIE；

（2）JIANGSHANGSHUHECHENGDEDNAPIANDUANKELONGZHIBAOGAOJIYINZAITIpsiCHECKZHILI；

（3）ZHIJIEHECHENGlncRNAXULIE，GOUJIANlncRNAGUOBIAODAZHILI；

（4）JIANGBAOGAOZAITIYUlncRNABIAODAZAITIGONGZHUANRAN293TXIBAO；

（5）DUOGONGNENGMEIBIAOYIJIANCEBAOGAOJIYINDEBIAODASHUIPING；

（6）SHUJUFENXI，ZHENGLITIJIAOKEHUYUANSHISHUJUJITIANBODIANJINGBAOGAO；

 ZHU：WEILESHIYANDEYANJINXING，KEJIARUBAJIYIN3’UTRQUmicroRNAYUCEJIEHEQUYUDETUBIANXINGBAOGAOZAITIJIlncRNAXULIEZHONGmicroRNAYUCEJIEHEQUYUDETUBIANXINGBIAODAZAITIZUOWEIDUIZHAO，JINYIBUSHUOMINGWENTI。

 

 

CANKAOWENXIAN：

 

Cesana, M., et al. (2011). "A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA." Cell 147(2): 358-369.