lncRNA/miRNA靶点验证

 

简介:

 

lncRNADEZUOYONGJIZHIYOUHENDUO,YIceRNA(competing endogenous RNA,JINGZHENGXINGDENEIYUANRNA)XINGSHIFAHUIGONGNENGSHIQIZHONGDEYIZHONG,JIlncRNAYUBAJIYINDE3’UTRJINGZHENGJIEHEXIANGTONGDEmicroRNA,JIANGDImicroRNADUIBAJIYINDEYIZHIGONGNENG,CONGERSHANGDIAOBAJIYINDEBIAODALIANG。WEILEJIANCEQIXIAOGUO,WOMENKEYIYONGWestern BlotJIANCEBADANBAIDEBIAODASHUIPING,YEKEYIYONGqRT-PCRJIANCEBAJIYINDEBIAODA。DANSHIZHEILIANGZHONGFANGSHIDOUWUFAQUEDINGlncRNASHIFOUTONGGUOceRNAFANGSHIDIAOKONGBAJIYINDEBIAODA。YINCI,WOMENKEYITONGGUO“microRNAYUlncRNAJIEHEYANZHENG”,“microRNAYUBAJIYINJIEHEYANZHENG”HE“lncRNAYUBAJIYIN3’UTRJINGZHENGJIEHEmicroRNAYANZHENG”SHIYAN,GOUJIANSensor reporterBINGJINXINGHUOXINGJIANCE,DUIlncRNA,microRNAYUBAJIYINZHEISANZHEJIANDEZUOYONGJIZHIZUOCHUZHUNQUEPANDUAN。

 

 

服务优势:

 

(1)LIYONGZHUANYERUANJIANJINXINGmiRNAYUlncRNAHEBAJIYIN3’UTRQUDEJIEHEWEIDIANYUCE;

(2)CAIYONGPromegaTIANBODIANJINGTEYOUpsiCHECKZHILI;

(3)SUOCAIYONGDELuciferaseSHUANGYINGGUANGBAOGAOXITONG,KEYIHUODEKUANFANDEXIANXINGDINGLIANGFANWEIYUJIGAODELINGMINDU;

(4)CAIYONGSHUNSHIZHUANRANJISHU,CAOZUOJIANBIAN、KUAISU,ZHOUQIDUAN,SHIYONGYUJUEDADUOSHUXIBAOLEIXING。

 

 

服务流程:

 

YI、microRNAYUlncRNAJIEHEYANZHENG

(1)ZHIJIEHECHENGBAlncRNAXULIEHUOmicroRNAZAIBAlncRNASHANGDEJIEHEWEIDIANXULIE;

(2)JIANGSHANGSHUHECHENGDEDNAPIANDUANKELONGZHIBAOGAOJIYINZAITIpsiCHECKZHILI;

(3)GOUMAImicroRNA mimics;

(4)JIANGBAOGAOZAITIYUmicroRNA mimicsGONGZHUANRAN293TXIBAO;

(5)DUOGONGNENGMEIBIAOYIJIANCEBAOGAOJIYINDEBIAODASHUIPING;

(6)SHUJUFENXI,ZHENGLITIJIAOKEHUYUANSHISHUJUJITIANBODIANJINGBAOGAO;

 ZHU:WEILESHIYANDEYANJINXING,KEJIARUBAlncRNAXULIEZHONGmicroRNAYUCEJIEHEQUYUDETUBIANTIZUOWEIDUIZHAO,JINYIBUSHUOMINGWENTI。

 

ER、microRNAYUBAJIYINJIEHEYANZHENG

(1)ZHIJIEHECHENGBAJIYIN3’UTRXULIEHUOmicroRNAZAIBAJIYIN3’UTRSHANGDEJIEHEWEIDIANXULIE;

(2)JIANGSHANGSHUHECHENGDEDNAPIANDUANKELONGZHIBAOGAOJIYINZAITIpsiCHECKZHILI;

(3)GOUMAImicroRNA mimics;

(4)JIANGBAOGAOZAITIYUmicroRNA mimicsGONGZHUANRAN293TXIBAO;

(5)DUOGONGNENGMEIBIAOYIJIANCEBAOGAOJIYINDEBIAODASHUIPING;

(6)SHUJUFENXI,ZHENGLITIJIAOKEHUYUANSHISHUJUJITIANBODIANJINGBAOGAO;

 ZHU:WEILESHIYANDEYANJINXING,KEJIARUBAJIYIN3’UTRQUmicroRNAYUCEJIEHEQUYUDETUBIANTIZUOWEIDUIZHAO,JINYIBUSHUOMINGWENTI。

 

SAN、lncRNAYUBAJIYIN3’UTRJINGZHENGJIEHEmicroRNAYANZHENG

(1)ZHIJIEHECHENGBAJIYIN3’UTRXULIEHUOmicroRNAZAIBAJIYIN3’UTRSHANGDEJIEHEWEIDIANXULIE;

(2)JIANGSHANGSHUHECHENGDEDNAPIANDUANKELONGZHIBAOGAOJIYINZAITIpsiCHECKZHILI;

(3)ZHIJIEHECHENGlncRNAXULIE,GOUJIANlncRNAGUOBIAODAZHILI;

(4)JIANGBAOGAOZAITIYUlncRNABIAODAZAITIGONGZHUANRAN293TXIBAO;

(5)DUOGONGNENGMEIBIAOYIJIANCEBAOGAOJIYINDEBIAODASHUIPING;

(6)SHUJUFENXI,ZHENGLITIJIAOKEHUYUANSHISHUJUJITIANBODIANJINGBAOGAO;

 ZHU:WEILESHIYANDEYANJINXING,KEJIARUBAJIYIN3’UTRQUmicroRNAYUCEJIEHEQUYUDETUBIANXINGBAOGAOZAITIJIlncRNAXULIEZHONGmicroRNAYUCEJIEHEQUYUDETUBIANXINGBIAODAZAITIZUOWEIDUIZHAO,JINYIBUSHUOMINGWENTI。

 

 

CANKAOWENXIAN:

 

Cesana, M., et al. (2011). "A long noncoding RNA controls muscle differentiation by functioning as a competing endogenous RNA." Cell 147(2): 358-369.
 

 

Chinese, Simplified
 
服务分类: