发高分文章,无支原体是前提!汉恒助力客户发表Cell Res

8YUE28RI,DALIANYIKEDAXUEZHONGLIUZHONGXINZHUREN、ZHONGLIUGANXIBAOYANJIUYUANYUANZHANGLIUQIANGJIAOSHOUYANJIUTUANDUIZAInatureZIKANCell Research(IF=20.507)SHANGFABIAOLUNZHU,WENZHANGBIAOTIWEI《Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells》,GAIYANJIUZHENGMINGDROSHAYUβ-CateninXIANGHUZUOYONG,JIHUOGANXINGJIYINSTC1,CONGERYOUZHUYUWEICHIRUXIANAIGANXIBAOYANGXIBAO(BCSC)DETEXING,ERAIJIYINAURKACUJINLEDROSHA mRNADE m6AXIUSHIBINGZENGQIANGLEQIWENDINGXING。

 

  ZHIDEYITIDESHI :

" HANHENGSHENGWUDEKANGZHIYUANTISHIJIanti-mycoplasma reagent SaveIt (Hanbio, HB-SV1000)BANGZHUTAWANCHENGLEZHIYUANTIWURANDEJIANCEHEQINGCHUGONGZUO."

 

XIAMIANJIUYIQILAIXIANGXILEJIEYIXIAZHEIPIANWENZHANGDENEIRONG~

 

 

BEIJINGJIESHAO

 

GENJU RNase III XINGDANBAIDEJIEGOUYUZUFEN,KEYIJIANGQIFENCHENGSANLEI;ERDROSHASHIDIERLEIRNase III XINGMEI。YIYOUYANJIUBIAOMINGYOUJIYINZUZHUANLUCHUDEQIANTImiRNA(pre-miRNA)ZAIXIBAOHENEIYOUDROSHAMEIJINXINGCHULI,CHENGWEIJUBEIJINGHUANJIEGOUDEpri-miRNA,ZAIExiportin-5DANBAIDEZHUANYUNZUOYONGXIACONGXIBAOHEZHUANYIZHIXIBAOZHIZHONG。DROSHAHAIKETONGGUOZHUANLUHOUKONGZHIRNAWENDINGXING,KEBIANJIANJIE,3'-MODUANJIAGONGHEZHUANLUZHONGZHIDENGDUOZHONGFANGSHIZHIJIEDIAOJIEZHENGGEZHUANLUZUDEGEZHONGRNADAIXIE。ZUIJINDEYANJIUFAXIAN,DROSHAZAIZHONGLIUZUZHIZHONGPUBIANGAOBIAODAQIEYUZHONGLIUDEBULIANGYUHOUXIANGGUAN,RANER,DROSHASHIRUHECUJINZHONGLIUFAZHANDEQIANZAIDEJIZHIHAIBUTAIQINGCHU。

 

 

SHIYANJIEGUO

 

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ROSHAYUβ-CateninXIANGHUZUOYONGJIHUOGANXINGJIYINSTC1,WEICHIRUXIANAIGANXIBAOYANGXIBAO(BCSC)TEXING

 

YANJIUZHESHOUXIANJIANCELEDROSHAZAIBCSCWEIHUZHONGDEZUOYONG,FAXIANBCSCZHONGDROSHADEmRNAHEDANBAISHUIPINGDOUYOUSUOSHANGDIAO;JINJIEZHEZUOLEDROSHAQIAODIHOUDEJIYINCHAYIXINGBIAODAFENXIHEGANXINGJIYINFENXI,FAXIANGRB10, SLCO4A1,SORBS2, STC1ZHEI4GEJIYINYOUXIANZHUXINGCHAYI,ZAITONGGUORT-qPCRJIANCEYEYANZHENGLEZHEIYIFENXIJIEGUO。ZUIHOU,FENBIEDIAODIZHEIJIGEJIYINFAXIANZHIYOU STC1 NENGGOUYINGXIANGALDH +YAQUNXIBAODESHULIANG,BIAOMINGSTC1DUIDROSHADIAOKONGDEBCSCsZHIGUANZHONGYAO。

 

 

 

 

 

ZAISHENRUYANJIUFAXIAN,STC1DEGUOBIAODAYOULIYUDROSHAQIAODIDERUXIANAIXIBAOALDH +YAQUNXIBAODEXINGCHENG,HUIFULEXIBAOQIUXINGDEXINGCHENGNENGLI,YEZENGJIALEDROSHAQIAODIXIBAOZHONGQIUXINGXIBAODEZHIJINGHESHULIANG;JIANGDROSHA QIAODIDEXIBAOHUODROSHA QIAODIDANSTC1GUOBIAODADEXIBAOZHURUXIAOSHUZHONGFAXIANJIEZHONGDROSHAQIAODIXIBAODESTC1GUOBIAODADEXIAOSHUBIZHUSHEDROSHAQIAODIXIBAODEXIAOSHUXINGCHENGGENGDADEZHONGLIU。